Fluorescein Isothiocyanate (Noun)
Meaning
A fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence.
Classification
Nouns denoting substances.
Examples
- Fluorescein isothiocyanate is a fluorochrome commonly used in various biological assays, including indirect immunofluorescence.
- The conjugation of fluorescein isothiocyanate to antibodies allows researchers to visualize specific proteins within cells.
- Fluorescein isothiocyanate is often used as a marker in immunofluorescence microscopy due to its high fluorescence intensity.
- The reaction between fluorescein isothiocyanate and the antibody's primary amine groups results in a stable conjugate suitable for indirect immunofluorescence.
- In indirect immunofluorescence, a primary antibody is first bound to the target protein, followed by a secondary antibody conjugated to fluorescein isothiocyanate.