Fluorescein Isocyanate (Noun)
Meaning
A fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence.
Classification
Nouns denoting substances.
Examples
- Fluorescein isocyanate is a popular fluorescent label that is often used to conjugate antibodies for immunofluorescence assays.
- The researchers conjugated the secondary antibodies with fluorescein isocyanate to visualize the protein of interest in the tissue samples.
- Conjugation of the primary antibody with fluorescein isocyanate was performed according to the manufacturer's instructions for optimal results.
- To detect the antigen, the researchers used an indirect immunofluorescence assay with a secondary antibody conjugated with fluorescein isocyanate.
- The high fluorescence yield of fluorescein isocyanate made it an ideal choice for conjugating the antibody used in the flow cytometry experiment.