Sticky End (Noun)
Meaning
An end of DNA in which one strand of the double helix extends a few units beyond the other.
Classification
Nouns denoting substances.
Examples
- The restriction enzyme cuts the DNA at a sticky end, allowing for ligation with another complementary sequence.
- A sticky end is generated when a restriction enzyme cuts the DNA, leaving an overhang that can bind to a complementary sequence.
- The cloning vector was designed with a sticky end that would allow researchers to easily insert new sequences.
- The DNA fragment had a sticky end that was complementary to the vector, making it easy to ligate into the plasmid.
- By cutting the DNA at a specific sticky end, researchers were able to isolate the gene of interest and amplify it using PCR.