Bromphenol Blue (Noun)
Meaning
A dye used as an acid-base indicator.
Classification
Nouns denoting substances.
Examples
- Bromphenol blue is often used as a tracking dye in DNA and RNA agarose gel electrophoresis, serving as a pH indicator to determine when a gel has reached a specific acidity.
- Bromphenol blue serves as a versatile acid-base indicator with its pH transition occurring from acidic to basic states within the yellow and blue range.
- Bromphenol blue acts as an effective indicator to show changes in the acidity levels by transforming colors ranging from yellow in an acidic solution to blue when moving to basic solution.
- Since the visible pH transition of bromphenol blue takes place at slightly acidic conditions, the pH level indicator primarily suits most commonly applied DNA isolation buffers.
- Scientists mix bromphenol blue as an indicator to loading buffers during various lab tests due to the contrasting and straightforward acidity based transformation the blue produces as opposed to commonly employed alkaline markers.